According to the ini_set function documentation, it is not possible to alter the value of register_globals on runtime.
EDIT: If you have register_globals = on in the php.ini, you can always use the superglobals arrays to access data: use $GET where you expect data to be input via the URL and use $POST where you expect data to be input via a form using method POST. That way, if you ever change the php.ini setting, you are already using the correct way to access the values input.