Nope. Unless you're loading PHP source files from different servers (I highly doubt this), no bandwidth is used in the processing of PHP files. The only bandwidth used is the incomming bandwidth of the client's "GET /myscript.php HTTP/1.1" HTTP request, and then the outgoing bandwidth of PHP outputting the HTML data.
Now, what you are dealing with is hard drive seek time (to read the file's contents), and memory/CPU (for PHP to parse all of that). If your files are that large and you notice slow process times or more CPU/memory usage than you'd like, you might try spliting up functions into different files. Try to group them together and label the file accordingly.