Well yes... kinda... lets say you have a class foo.
Foo describes can describe an object that has a size and shape field. Foo also has functions that can operate on these fields as well as do other things... so you might say this:
require("foo.inc"); // or class file
// Create a new object named object
$object = new Foo();
// Set our fields
$object->setSize(3);
$object->setShape("Circle");
// Get and echo fields
echo $object->getSize();
echo $object->getShape();
So yes you do have to include the class in the file... but in order to use the class... you need to make a new object of that class. I hope that helps.